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. Author manuscript; available in PMC: 2015 Dec 30.
Published in final edited form as: Free Radic Biol Med. 2003 Nov 1;35(9):1064–1072. doi: 10.1016/s0891-5849(03)00468-4

Fig. 3.

Fig. 3

Age-related increase in the 4-HNE modified mitochondrial electron transport chain. Blue native gel electrophoresis (BN-PAGE) was performed to separate the mitochondrial electron transport chain complexes from young (n = 4) and old animals (n = 4). Proteins were transferred to PVDF membranes and were probed with polyclonal rabbit anti-sera as described. Results show a marked age-related increase in the steady state levels of 4-HNE modified Complex IV in IFM isolated from old compared to young. Results are expressed as % increase relative to young IFM.