Figure 5.
LA supplementation leads to marked reduction in the steady-state levels of 8-oxo-dG. The steady-state levels of 8-oxo-dG in freshly isolated hearts from old and young rats after 2 wk supplementation with an AIN-93M diet with or without 0.2% (w/w) (R)-α-lipoic acid by using coulometric detection after separation with HPLC. The results show that LA supplementation significantly lowered the age-related accumulation of 8-oxo-dG in hearts from old rats. Results are expressed as mean ± SE for heart isolated from young (n=8), young LA (n=3), old (n=10), and old LA (n=9). One-way ANOVA was used for statistical analysis with the level of significance defined as P < 0.05.