Fig. 1.

Genetic deletion of the PGE₂ EP3 receptor attenuates ICH-induced brain injury. At 72 h after ICH, Cresyl violet staining of coronal brain sections from WT and EP3^−/− mice was performed to evaluate brain injury. (A) Representative images showing characteristic hematoma profiles for WT (upper panels) and EP3^−/− mice (bottom panels). Images are from the same animal, where left to right corresponds to anterior to posterior and center images are at the needle insertion site, representing maximal brain injury. (B) Lesion volume quantification demonstrated that EP3^−/− mice had significantly less brain injury following ICH. (C) Red/brown positive pixel count analysis showed that EP3^−/− mice had significantly less blood accumulation within the injured brain areas. (D) No significant difference in percent ipsilateral hemispheric enlargement was seen between the groups. All comparisons included n = 7 WT and n = 8 EP3^−/− mice, ns = not significant and **p < 0.01.