Fig. 6.

Genetic deletion of the PGE₂ EP3 receptor reduces peripheral neutrophil infiltration after ICH. Immunohistochemical staining for MPO was used to evaluate peripheral neutrophil infiltration in WT and EP3^−/− mice 72 h following ICH. (A) Representative high magnification images of coronal brain sections showing MPO+ cells (brown) diffusely localized within injured brain regions. Square selections in the inserts denote magnified regions. (B) Brown positive pixel count analysis of the ipsilateral hemisphere showed that EP3^−/− mice had significantly less peripheral neutrophil infiltration (WT: n = 5, EP3^−/−: n = 8, *p < 0.05). No MPO+ cells were seen outside of the injured brain areas for any of the mice in the study.