Fig. 7.

Genetic deletion of the PGE₂ EP3 receptor attenuates blood brain barrier breakdown after ICH. Immunohistochemical staining for mouse IgG was used to evaluate BBB breakdown in WT and EP3^−/− mice at 72 h following ICH. (A) Representative images of coronal brain sections showing more disruption of the BBB in WT mice, which displayed a more diffuse staining pattern. Square boxes denote the location of high magnification inserts. (B) Whole brain brown positive pixel count analysis showed that EP3^−/− mice had significantly reduced BBB breakdown (WT: n = 6, EP3^−/−: n = 7, **p < 0.01).