Figure 7.

Sinefungin inhibits UUO-induced upregulation of H3K4me1 in UUO mice concomitantly with the amelioration of renal fibrosis in obstructive nephropathy. The same protein lysates as in Figure 6 were used. (A) Representative western blot analysis showing the levels of H3K4me1, (B) H3K4me2, and (C) H3K4me3 protein expression in UUO mice with or without sinefungin injection (10 mg/kg). Quantification is shown in the right panel (n=5 for each group). Data were analyzed by one-way ANOVA followed by the post hoc test using t test with Bonferroni correction. ^##P<0.01. Non-op CTL, non-operated normal controls; Sine, sinefungin; Veh, vehicle.