Skip to main content
. 2015 May 14;27(1):79–90. doi: 10.1681/ASN.2014101030

Figure 1.

Figure 1.

The kidney contributes to circulating blood αKlotho. (A) αKlotho protein levels in inferior vena cava (IVC) in normal SD rats. Upper panel shows immunoblot of αKlotho protein immunoprecipitated with anti-αKlotho antibody from 0.1 ml serum from infrarenal and suprarenal IVC. Different amounts of recombinant full-length murine αKlotho protein (rMKl) in left three lanes. Bottom panel show four independent animals with each one connected by lines. Means±SD are shown as circles and error bars. Statistical significance was assessed by paired t test, and accepted when **P<0.01. (B) αKlotho protein levels in human IVC. Upper panel shows immunoblot of αKlotho proteins immunoprecipitated with anti-αKlotho antibody from 0.1 ml serum from infrarenal and suprarenal IVC from three subjects. Bottom panel is a summary from nine patients and significant difference between infrarenal and suprarenal IVC was assessed by paired t test, and significant differences were accepted when **P<0.01. (C) Serum αKlotho levels (y axis) were plotted against serum erythropoietin (EPO) levels (x axis) measured in the same sample. The infrarenal and suprarenal samples from the same individuals are connected by lines. (D) Change in endogenous serum αKlotho in anephric rats subjected to bilateral nephrectomy (Npx). Blood was drawn at the specified time points post-surgery. Serum αKlotho was determined by immunoprecipitation-immunoblot. Upper panel is a representative blot. Bottom panel is a summary of serum αKlotho and serum Cr from three independent experiments. Data are expressed as means±SD. Statistical significance was assessed by one-way ANOVA followed by Student–Newman–Keuls test, and significant differences were accepted when *P<0.05; **P<0.01* versus hour 0. (E) WT mice were injected intraperitoneally with α- or/and β-secretase inhibitors (α-Inh, β-Inh) for 2 days and serum αKlotho was determined by immunoprecipitation-immunoblot. Upper panel is one set of representative data. Bottom panel is a summary of arbitrary unit of serum αKlotho over IgG HC from three independent experiments. Statistical significance was assessed by one-way ANOVA followed by post hoc Student–Newman–Keuls test, and was considered significant when *P<0.05 or **P<0.01 versus vehicle (DMSO); #P<0.05 versus α-secretase inhibitor.