Figure 3.

Tissue distribution of exogenous recombinant mouse αKlotho in normal rats. ¹²⁵I-αKlotho and ¹²⁵I-albumin were intravenously injected into normal Sprague-Dawley rats and blood, urine, and organs were collected. (A) Representative autoradiographs of organs harvested from rats injected with ¹²⁵I-αKlotho and ¹²⁵I-albumin. (B) Radioactivity of ¹²⁵I-αKlotho and ¹²⁵I-albumin in organs normalized to protein concentration (left panel), and of ¹²⁵I-αKlotho and ¹²⁵I-albumin in serum and urine (right panel). Data summarized from four independent experiments. (C) ¹²⁵I-labeled αKlotho protein was intravenously injected into normal rats and kidneys were harvested at specific time points and cryosections were made for autoradiography. (D) Infrared-labeled αKlotho was intraperitoneally injected into normal rats. Two hours later, kidney, spleen, and heart were harvested, sectioned, and scanned with IR imager system.