Figure 5.

Intact exogenous αKlotho is present in blood and urine. ¹²⁵I-αKlotho and ¹²⁵I-albumin were intravenously injected into normal rats and blood and urine were collected 1 and 2 hours after injection, respectively. (A) ¹²⁵I-αKlotho and (B) ¹²⁵I-albumin in urine and serum were visualized on SDS-PAGE. (C) Urine from free-flow micropuncture originating from PCT, Bowman’s space, bladder urine, and serum of rats injected with ¹²⁵I-αKlotho or ¹²⁵I-albumin, were collected at 1 hour after injection and the same volumes were subjected to scintillation counting.