Table 1.

Primer sequence for real-time quantitative RT-PCR

Gene	Sense	Antisense
Lcn-2	5′-CAGAAGGCAGCTTTACGATG-3′	5′-CCTGGAGCTTGGAACAAATG-3′
Lysozyme	5′-CCAGTGTCACGAGGCATTCA-3′	5′-TGATAACAGGCTCATCTGTCTCA-3′
Il-6	5′-GAGGATACCACTCCCAACAGACC-3′	5′-AAGTGCATCATCGTTGTTCATACA-3′
Il-8 (KC)	5′-TGTCAGTGCCTGCAGACCAT-3′	5′-GAGCCTTAGTTTGGACAGGATCTG-3′
Tnfa	5′-CATCTTCTCAAAATTCGAGTGACAA-3′	5′-TGGGAGTAGACAAGGTACAACCC-3′
Tgfb	5′-CACCTGCAAGACCATCGACAT-3′	5′-GAGCCTTAGTTTGGACAGGATCTG-3′
p21	5′-CCCTCTATTTTGGAGGGTTAATCT-3′	5′-GTACCCTGCATATACATTCCCTTC-3′
Nephrin	5′-AGGATCGAATCAGGAATGAAT-3′	5′-CTGTGAAGGCTTGGCGATATGACA-3′
Podocin	5′-GTGTCCAAAGCCATGCAGTT-3′	5′-GCAATGCTCTTCCTTTCCAG-3′
p53	5′-ACTGCATGGACGATCTGTTG-3′	5′-GCCATAGTTGCCCTGGTAAG-3′
Wt1 (+KTS)	5′-GAGACACACAGGTGTGAAACCATTC-3′	5′-GACAGCTGAAGGGCTTTTCACTTG-3′
Wt1 (total)	5′-GAGACACACAGGTGTGAAACCATTC-3′	5′-GCGCCACGTGGAGTTTGGTCATG-3′
Gapdh	5′-CCTGGAGAAACCTGCCAAGTATG-3′	5′-GGTCCTCAGTGTAGCCCAAGATG-3′

Gapdh, glyceraldehyde-3-phosphate dehydrogenase.