Figure 10.

The synergistic effect of CGS21680/LPS against renal IRI in WT, HIF-2α^−/− and Mx1-HIF-2α^−/− mice. CGS21680 (0.7 mg/kg) and LPS (3 mg/kg) were administered intraperitoneally together. Twenty-four hours later, the mice were subjected to right nephrectomy and 25 minutes of ischemia in the left kidneys, followed by 24 hours of reperfusion. (A) Serum creatinine concentrations (n=6 per group). CGS21680/LPS treatment failed to reduce creatinine level in Mx1-HIF-2α^−/− mice (P>0.05 versus vehicle-treated mice). *P<0.05 versus vehicle-treated controls. Representative (B) PAS, (C) MPO staining sections and (D) TUNEL assay in post-ischemic kidneys harvested at 24 h after reperfusion. Original magnification, ×200. MPO, myeloperoxidase; PAS, periodic acid–Schiff.