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. 2015 May 8;27(1):92–106. doi: 10.1681/ASN.2014121248

Figure 4.

Figure 4.

In vivo NK1.1+ cell depletion eliminated the difference between HIF-2α−/− mice and WT littermates. Mice were injected intraperitoneally with 250 μg anti-NK1.1 monoclonal antibody (PK136) or isotype-matched control IgG2a at 48 hours before ischemic insult (20 minutes). (A) NK1.1+ cell depletion was confirmed by flow cytometry analysis. (B) Serum creatinine levels at 24 hours after the reperfusion (n=6 per group). *P<0.05 versus isogenic mice treated with control IgG. (C) Survival of mice after PK136/control IgG pretreatment and 20 minutes of renal ischemia (n=10 per group). Compared with control IgG, PK136 led to a significant survival advantage in HIF-2α−/− mice by Kaplan-Meier analysis (log-rank test, P<0.05). (D) Representative PAS-stained sections in post-ischemic kidneys harvested at 24 hours. Original magnification, ×200. PAS, periodic acid–Schiff.