Figure 7.
Adoptive transfer of HIF-2α−/− NKT cells elicited more severe renal IRI to Rag1KO mice than WT NKT cells, and HIF-2α−/− NKT cells were not responsive to CGS21680 treatment. NKT cells were isolated in a two-step procedure from HIF-2α−/− and WT mouse spleens. About 3×105 NKT cells were adoptively transferred into one Rag1KO mouse via tail vein injection at 4 days before CGS21680 treatment and renal IRI. (A) Serum creatinine concentrations at 24 hours after reperfusion (n=4 per group). Statistical significance or insignificance is indicated. (B) Representative PAS-stained renal sections from mice subjected to 20 minutes of ischemia and 24 hours of reperfusion are shown. Original magnification, ×200. Abnormalities based on PAS-stained sections were graded and data are expressed as mean±SD from four mice per group. Statistical significance or insignificance is indicated. PAS, periodic acid–Schiff.