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. Author manuscript; available in PMC: 2016 Jul 27.
Published in final edited form as: ACS Infect Dis. 2015 Jul 27;1(8):340–349. doi: 10.1021/acsinfecdis.5b00070

Figure 3.

Figure 3

Dose–response inhibition of the N7 and 2′-O methylation activities of the WNV MTase by the most potent compound, NSC 12155. (A) TLC analyses of the N7 and 2′-O inhibition of the WNV MTase by NSC 12155. The migration positions of the G*pppA and m7G*pppA molecules are labeled on the side of the TLC images. (B, C) Curve fitting to determine the IC50 values for each compound on the N7 (B) and 2′-O (C) MTase activities of the WNV MTase. The percentage of activity was determined after quantification of G*pppA and m7G*pppA. The IC50 value was determined by fitting of the dose–response curve as described under Materials and Methods. Each reaction was carried out in triplicate, and the standard deviation is plotted. (D) Analysis of the effects of CHAPS on the N7 methylation activity of the WNV MTase.