Skip to main content
. Author manuscript; available in PMC: 2016 Jul 27.
Published in final edited form as: ACS Infect Dis. 2015 Jul 27;1(8):340–349. doi: 10.1021/acsinfecdis.5b00070

Figure 7.

Figure 7

NSC 12155 interactions with the MTase. (A) Dose response of inhibition of the [3H]-SAM–MTase complex formation by NSC 12155. The biotinylated DENV3MTase and 3H-labeled SAM are incubated with or without NSC 12155. A 2-fold dilution series is shown. Streptavidincoated SPA beads are used to mix with the reaction mixtures, and the SAM-binding is quantified using a Microbeta2 scintillation counter. (B) Cartoon representation of the crystal structure of the DENV3MTase–12155 complex. NSC 12155 is in stick representation. Atom colors (unless otherwise specified): carbon, yellow; nitrogen, blue; and oxygen, red. (C) NSC 12155 interaction with the DENV3MTase residues in stick representation, with carbon in cyan color. The omit Fo−Fc electron density map for NSC 12155, contoured at 2δ level, is shown in gray. (D) Superimposition of the structure of the MTase–12155 complex (cyan) with that of SAM-free MTase (gray) (PDB 4R05)11 and that of MTase–SIN complex (pink) (PDB 4R8S).17 Residues interacting with NSC 12155 are shown in stick representation. The carbon atom of SIN is in green.