Fig 4. Suppression of AQP1 transcription by TNF-α is restrained by KLF2.

HUVECs were transfected with either empty lentiviral vector (mock) or with a lentiviral vector carrying KLF2 under control of the PGK promoter (KLF2) and two days after transfection incubated with vehicle (ctr) or TNF-α at a concentration of 20 ng/ml during 24 hours (TNF). mRNA levels were determined for AQP1, normalized to P0 and expressed as mean and SEM (N = 4). *P<0.05, **P<0.01.