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. 2015 Dec 30;10(12):e0146099. doi: 10.1371/journal.pone.0146099

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© 2015 Graumann et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Immunohistochemical staining of CK-19 (grey) shows that HBs expression (red) in every hepatocyte went along with higher numbers of single CK-19⁺-oval cells around periportal fields. Scale bar 32 μm. Arrow: CK-19 positive bile duct epithelium, arrowheads: single CK-19 positive cells in parenchyma. (B) Immunohistochemical staining of Sox-9 (black) indicates that areas with HBs expression (red) exhibited more Sox-9⁺-oval cells in periportal tracts and surrounding parenchyma than areas without HBs expression. Scale bar 80 μm. Arrow: Sox-9 positive cells around a bile duct, arrowhead: Sox-9 positive cells in parenchyma.