Table 1. Automated purification of rLDHA.

Fraction	Total protein (mg) a	Purity (%) b
Soluble clarified lysate supernatant	248	~ 13
Pooled HiLoad Superdex200pg 26/60 fractions.	14	≥ 93

Fractionation was performed on cell pellets obtained from E. coli cultured in 50 ml of EnPresso B media, which generates the equivalent biomass of 1 L of traditional liquid LB media.

^a Mean values from at least 2 individual repeat runs. Protein concentration in the supernatant and the pooled fractions was determined by A280 measurements.

^b average % purity determined by densitometry of appropriate lanes on reducing SDS-polyacrylamide gels (4–20% gradient, see Fig 1A).