Skip to main content
. 2015 Dec 30;11(12):e1005649. doi: 10.1371/journal.pgen.1005649

Fig 2. Auxin and ethylene responsiveness of SlARF2A and SlARF2B genes.

Fig 2

(A) qPCR analysis of SlARF2A and SlARF2B transcripts in total RNA samples extracted from wild-type mature green fruits treated with 50 ml.L-1 ethylene for 5 hours. (B) qPCR analysis of SlARF2A and SlARF2B transcripts in total RNA samples extracted from wild-type breaker fruits treated with 1-MCP (1.0 mg.L-1) for 16 hours. (C) qPCR analysis of SlARF2A and SlARF2B transcripts in total RNA samples extracted from wild-type mature green fruits treated with 20 μM IAA or buffer (control) for 6 hours. The relative mRNA levels of SlARF2A/SlARF2B genes were normalized against actin. The results were expressed using control untreated fruit as reference with relative mRNA level set to 1. Error bars mean ±SD of three biological replicates. Stars indicate the statistical significance using Student’s t-test: *0.01 < p-value < 0.05, ** 0.001< p-value <0.01, *** p-value < 0.001. E4, E8: ethylene response genes; GH3, SAUR: auxin response genes.