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. 2015 Dec 30;11(12):e1005649. doi: 10.1371/journal.pgen.1005649

Fig 8. The expression of ethylene synthesis and ethylene perception genes is altered in SlARF2AB-RNAi plants.

Fig 8

(A) Expression of ethylene synthesis pathway genes in SlARF2AB-RNAi lines assed by Quantitative RT-PCR. ACO1, ACO2, ACO3, ACO4 aminocyclopropane-1-carboxylic acid oxidase; ACS1, ACS2, ACS3, ACS4, ACS6 aminocyclopropane-1-carboxylic acid synthases. (B) Expression of ethylene perception genes in SlARF2AB-RNAi assessed by Quantitative RT-PCR. EIN2 ethylene signaling protein; EIL2 and EIL3 are EIN3-like proteins; ETR1, ETR2, ETR3 (NR, never-ripe), ETR4, ETR5, ETR6 ethylene receptors; CTR1 ethylene-responsive protein kinase. ABL1 refers to SlARF2AB-RNAi line 311. Total RNA was extracted from different fruit developmental stages (breaker, Br; Br+2, 2 days post-breaker; Br+8, 8 days post-breaker). The relative mRNA levels of each gene in WT at the breaker (Br) stage were standardized to 1.0, referring to the SlActin gene as internal control. Error bars mean ±SD of three biological replicates. Stars indicate statistical significance using Student’s t-test: * p-value<0.05, ** p-value<0.01.