Table 1. Primers for PCR amplification of various segments of the UGT1A1 gene.

Gene region amplified	Sequence (5’ to 3’)	PCR product size (bp)	Reference
Promoter region (for fragment length analysis)	6-FAM—TAA CTT GGT GTA TCG ATT GGT TTT TG	90	[9]
	ACA GCC ATG GCG CCT TTG CT
Promoter region (for sequencing)	AAG TGA ACT CCC TGC TAC CTT	253	[10]
	CCA CTG GGA TCA ACA GTA TCT
Regulatory region	CTA GCC ATT CTG GAT CCC TTG	367	[9]
	TTT TGA GAT CTG AGT TCT CTT CAC CTC
Exon 1*	TAT AAG TAG GAG AGG GCG AAC C	588	[9]
	TCA AAT TCC AGG CTG CAT G
	GGC CTC CCT GGC AGA AAG	617	[9]
	ATG CCA AAG ACA GAC TCA AAC C
Exon 2	TCT ATC TCA AAC ACG CAT GCC	374	[9]
	GGC AGG GAA AAG CCA AAT CTA
Exon 3	TTG CCA GTC CTC AGA AGC CTT	423	[9]
	ATG CCC TTG CAG AAA TTT GC
Exon 4	TGC AAG GGC ATG TGA GTA ACA	553	[9]
	AAG CCA AGA TTG CAC CAC TG
Exon 5	GAG GAT TGT TCA TAC CAC AGG	436	[8]
	TGA ATT TAA CAC TGA TTC TGT T
Primers for generating pCAS2 mini-gene constructs
UGT1A1-Ex3-BamHIF	GCATGCGGATCCCGGAAGTTGCCAGTCCTCAG	475
UGT1A1-Ex3-MluIR	CTAAGTACGCGTGTGTTACTCACATGCCCTTGC
Primers for pCAS2 RT-PCR analysis
pCAS_P1_F	CTCCGCAGGTCCGCT		[11]
pCAS_P2_R	ATTGGTTGTTGAGTTGGTTGTC

6-FAM = 6-carboxyfluorescein

*Exon 1, being long, was amplified as two overlapping amplicons; the sequences obtained from these were then merged