Fig 11. A portion of the maximum likelihood tree of carabids for CAD with all contigs included from the de novo assembly.

An example in which our BLAST searches for target genes within HTS museum specimen assemblies returned multiple contigs, however our filtering criteria failed to accept a best contig, despite two contigs falling in the prediction group (shown in pink), and being nearly identical to the PCR-based sequence of a conspecific specimen (Bembidion “Inuvik” 3984).