A, Representative images (40 X) showing (left to right) immunofluorescence for intracellular α4F (488 λ) and endoplasmic reticulum (ER, 546 λ) after 48 h treatment of transfected HEK-293 cells with GABA + THP with or without flumazenil (FLU, 10 μM). (Control, 0.01% DMSO) 48 h FLU (lower panel) increased intracellular α4F. B, Averaged data of the mean intracellular α4F intensity. (n=9, *F(2,6)=162.5, P<0.0001, ANOVA; P<0.05 vs. other groups, post-hoc Tukey’s test). Scale, 10 μm.