Fig. 4.

The diagram illustrates the kisspeptin-ir area in the Arc from 36 days old wild type male (WT/CAS) and female (WT/OVX) mice gonadectomized at P21 and SF-1 KO male (KOM) and female (KOF) mice. All mice were untreated or treated with EB from P25 to P36. EB treatment from P25 to P36 had no effect on the kisspeptin-ir area in both WT/CAS and WT/OVX mice when compared to untreated gonadectomized WT mice. However, EB treatment did eliminate the sex difference in agonadal SF-1 KO mice at P36 by moderately (nonsignificantly) increasing immunoexpression in males and decreasing immunoexpression in females. The kisspeptin-ir area is presented as mean +/- SEM. *p< 0.05 (different from WT/CAS).