Fig. 3.
Expression of the 2-Cys Peroxiredoxins and 2-Cys Peroxiredoxins-SO2/3 proteins in the ovaries of mice following stimulation with PMSG/hCG. (A) Western blot analysis of the protein expression levels of β-actin (loading control), Prx1, Prx2, Prx3, as well as Prx1-, 2-, and 3-SO2/3 in the ovaries of ICR mice subjected to single (×1) or repeated (×3) PMSG/hCG injections. Ovaries were harvested 12 h after the final dose of PMSG/hCG. Tissue collection of the untreated mice (normal) was restricted to the estrus/metestrus stage of the natural estrous cycle.(B) Quantification of the relative protein expression levels of Prx1, 2, and 3 as well as Prx1-, 2-, and 3-SO2/3 normalized to β-actin expression. Data in the bar graphs represent means ± SEM of three independent experiments. **p < 0.01, ***p < 0.001 compared with normal.