FIGURE 6.

Thyroid hormone (T₃) induced FOXO1 target genes in an AKT-dependent manner. A, Western blot analysis for phosphorylated AKT and FOXO1 in HepG2 cells. LY292004 compound (5 μm) was used to inhibit AKT in HepG2 cells along with or without T₃ (100 nm). Bar graph represents relative densitometric measurements of phosphorylated AKT and FOXO1. Statistical significance was calculated as *, p < 0.05, and error bars represent mean ± S.D. B, transcript expression of PCK1 and IGFBP1 in HepG2 cells treated with LY292004 compound and/or T₃. Statistical significance was calculated as *, p < 0.05, and error bars represent mean ± S.D. C, Western blot analysis of myristoylated AKT (myrAKT), a constitutively active form of AKT, overexpression in THRB1-HepG2 cells. Bar graph represents relative densitometric measurements of total as well as phosphorylated AKT. Statistical significance was calculated as *, p < 0.05, and error bars represent mean ± S.D. D, transcript expression of PCK1 and IGFBP1 in HepG2 cells overexpressing myrAKT and treated with or without T₃. Statistical significance was calculated as *, p < 0.05, and error bars represent mean ± S.D. ACTB, β-actin.