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. 2015 Nov 6;291(1):244–254. doi: 10.1074/jbc.M115.673400

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FIGURE 5. — Adsorption of CB2_PH to PI(3)P. A, characteristic time trace of a RIfS experiment showing the specific binding of CB2_PH to a PI(3)P-containing membrane. Different concentrations of CB2_PH were added at time points as indicated by arrows (a, 0.038 μm; b, 0.11 μm; c, 0.19 μm; d, 0.26 μm; e, 0.37 μm; f, 0.74 μm). After rinsing the system with buffer A (g), a fraction of the bound protein desorbs from the membrane, indicating reversible binding. The inset shows the determination of the saturation value d_protein. B, adsorption isotherm obtained from the data shown in A. The solid gray line is the result of fitting a Langmuir isotherm (Equation 1) to the data with d_protein-max = 0.69 ± 0.04 nm and K_D = 0.09 ± 0.02 μm.