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Dissociation constants (KD) for the binding of CB2PH, CB2SH3−, and CB2SH3+/W24A/E262A to PIP-containing solid-supported membranes
|
KD |
||||||
|---|---|---|---|---|---|---|
| PI(3)P | PI(4)P | PI(3,4)P2 | PI(3,5)P2 | PI(4,5)P2 | PI(3,4,5)P3 | |
| μm | ||||||
| CB2PH | 0.10 ± 0.01 | 0.16 ± 0.03 | 0.8 ± 0.2 | 0.60 ± 0.06 | 0.4 ± 0.1 | |
| CB2SH3− | 0.32 ± 0.08 | 0.26 ± 0.06 | 1.2 ± 0.5 | 0.63 ± 0.09 | 0.3 ± 0.1 | |
| CB2SH3+/mutant | 0.27 ± 0.02 | 0.25 ± 0.04 | 0.9 ± 0.1 | 1.9 ± 0.2 | ||
