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PIAS3 is dispensable for ATRIP SUMOylation. HeLa cells were transfected with control siRNA or siRNAs targeting the PIAS family ligases. Endogenous ATRIP was immunoprecipitated under a denaturing condition. The unmodified and SUMOylated forms of ATRIP were detected with ATRIP and SUMO-2/3 antibodies, respectively.
