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Mutation of sbnI results in altered expression throughout the sbn operon. RN6390 and its sbnI::Tet derivative were grown in cTMS with 0.1 μm EDDHA, a medium in which growth was comparable between the two strains. qPCR was used to measure RNA levels for each gene, where WT gene expression was set to 1. Statistics were performed using the Students unpaired t test. *, p < 0.005.
