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. 2015 Nov 6;291(1):334–341. doi: 10.1074/jbc.M115.685289

FIGURE 3.

FIGURE 3.

Cooperative interaction between STIM1 and the N and C termini of Orai1 controls channel activation. A, representative plots of the current-voltage relationship of currents recorded from cells expressing STIM1 with mCherry-Orai1 or with the indicated mCherry-Orai1 mutants. pF, picofarad. B, summary of current densities recorded from cells expressing STIM1 with the indicated single or double mCherry-Orai1 mutations (n = 10–15 cells). C and D, representative fluorescence images (C) and summary of fluorescence densities (D) measured from cells expressing the WT or the W76C/L276C Orai1 double mutant (n = 6 regions in each). E, representative plots of the current-voltage relationship of currents recorded from cells expressing WT Orai1-S or Orai1-S with the indicated Orai1 mutation. F, summary of current densities recorded from cells expressing Orai1-S with the indicated single or double mutations (n = 6–9 cells). G and H, representative fluorescence images (G) and summary of fluorescence densities (H) measured from cells expressing the WT Orai1-S or the W76C/L276C Orai1-S double mutant (n = 6 regions in each). Dashed red columns show the predicted additive effect for the double mutants. Scale bars = 10 μm. *, p < 0.05; **, p < 0.01.