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Spectroscopic characterization of MrHR. A, absorption spectral red shift by the Cl− binding to the unphotolysed state. The medium (pH 6.5) contained 33 mm Na2SO4, 0.05% DDM, and NaCl (0–100 mm). B, titration with various anions, which were added by sodium salts. The relative absorbance changes at 550 nm are plotted. C, flash-induced absorbance changes at selected wavelengths. The medium (pH 6.5) contained 0.05% DDM and 100 mm NaCl. mOD, milli-optical density.
