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A model for sequential binding of Dna2 to Mec1. A, scheme depicting random or sequential binding of Dna2 activator to Mec1. The random pathway is inconsistent with the data below. B, titration of Dna2(1–499) in an assay with or without Dna2(1–499)WY-AA. C, titration of Dna2(1–499)WY-AA in assays with subsaturating concentrations of the indicated proteins, or the Ddc1-1 or Dna2-1 peptide.
