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. 2015 Nov 17;291(1):413–424. doi: 10.1074/jbc.M115.686709

FIGURE 4.

FIGURE 4.

Enhanced palmitate-induced, pro-inflammatory cytokine production under hypoxia is HIF-1α- and HIF-2α-independent. A–F, mRNA expression levels of HIF-1α (A), HIF-2α (B), the HIF target gene GLUT1 (C), IL-6 (D), IL-1β (E), and IL-8 (F) in macrophages transfected with control, HIF-1α, HIF-2α, or HIF-1α/HIF-2α siRNA and treated with 500 μm palmitate (C16:0) for 9 h under normoxia (20% O2) or hypoxia (1% O2). G–I, protein concentrations of IL-6 (G), IL-1β (H), and IL-8 (I) in macrophages transfected with control, HIF-1α, HIF-2α, or HIF-1α/HIF-2α siRNA and treated with 500 μm C16:0 for 24 h under normoxia (20% O2) or hypoxia (1% O2). Results are presented as mean ± S.E. of at least three independent experiments. C–F, the expression in hypoxic C16:0-treated control siRNA macrophages was set to 1. *, p < 0.05.