TABLE 1.
Growth of mutants that suppress FAD mutations of PS1 (L166P and G384A)
| Mutants | Growtha | No. of clones isolated |
|---|---|---|
| L166P, PCR mutation | ||
| Revertants | ||
| L166 (true revertant) | +++ | 104 |
| L166T | +++ | 34 |
| L166S | ++ | 60 |
| L166H | ++ | 2 |
| Second mutation | ||
| K380E/L232H/C419G | +++ | 1 |
| K380E/V272I | ++ | 1 |
| Directed mutation | ||
| L232H/C419G | − | |
| K380E/L232H | ++ | |
| K380E/C419G | ++ | |
| K380E | + | |
| C419G | − | |
| L232H | − | |
| V272I | − | |
| G384A, PCR mutation | ||
| Revertants | ||
| G384 (true revertant) | +++ | 2 |
| Second mutation | ||
| S438P | + | 12 |
| S438P/I287L | ++ | 1 |
| L432M/C158Y | ++ | 1 |
| Directed mutation | ||
| I287L | − | |
| L432M | + | |
| C158Y | − | |
a The growth of cells with PS1 mutations (L166P and G384A) were analyzed on SD-LWHUAde medium after 3 days at 30 °C. All cell contains NCT, Aph-1aL, Pen2, and APPC55-Gal4p. +++ represent wild-type growth, formed >1 mm colonies and − represents no growth. ++ or + represents partial growth, formed small colonies (>0.5 or <0.5 mm, respectively). 9.5 × 105 or 1.1 × 106 cells were screened for L166P or G384A suppressors, respectively.