Fig. 4.

a Colonization of WT and wrky6 Arabidopsis roots by P. indica after co-cultivation under 0.25 mM and 2.5 mM Pi concentrations in the media. The fungal material was stained with Trypan Blue. Photos are representative for more than 10 repetitions. b Quantification of the degree of root colonization by quantitative RT-PCR. The amount of fungal material (determined as fungal DNA with P. indica-specific primers) is expressed relative to the plant GAPDH DNA (determined with GAPDH-specific primers). Graphs are based on 3 independent experiments with 20 plants each. Bars represent SEs. Asterisk indicates significant difference, as determined by Student’s paired t-test for two tailed distribution (* P ≤ 0.05)