Fig. 4.

Flow cytometry characterization of terminal differentiation of CD34⁺CD31⁺ endothelial progenitors to venous and arterial endothelial cells under serum-free conditions. a Schematic representation of differentiation of hESCs to endothelial progenitors and further differentiation towards venous ECs (hESC-Ven-ECs) and arterial ECs (hESC-Art-ECs). b Representative flow cytometry histogram overlays represent the expression of pan-endothelial, arterial and venous markers among H1-Ven-ECs, H1-Art-ECs, H9-Ven-ECs, H9-Art-ECs, HUVECs, and HCAECs. bFGF Basic fibroblast growth factor, BMP4 Bone morphogenetic protein 4, EGF Epidermal growth factor, GSKi Glycogen synthase kinase inhibitor (CHIR99021), HCAEC Human coronary artery endothelial cells, hESC Human embryonic stem cells, HUVEC Human umbilical vein endothelial cells, VEGF Vascular endothelial growth factor