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. 2015 Dec 30;15:114. doi: 10.1186/s12896-015-0229-6

Fig. 6.

Fig. 6

Fluorescence images of immunocytochemical staining of DF stem cells induced for osteogenic differentiation using simple osteogenic medium in upper panel: (a) osteocalcin (OC FITC), (b) osteonectine (ON FITC), (c) osteopontin (OP FITC) and (d) alkaline phosphatase (ALP FITC). In the lower panel are illustrated the induced expression of same osteogenic markers when DF stem cells were cultivated in presence of complex osteogenic medium: (e) OC FITC, (f) ON-FITC, (g) OP-FITC and (h) ALP-FITC. Nuclei were counterstained with DAPI (Magnification ×400)