Table 2.

Formula used for simple and complex osteogenic mediums

Simple osteogenic differentiation medium (OS)	Complex osteogenic differentiation medium (OC)
• DMEM high glucose:F12 HAM(1:1) • 10 % fetal calf serum • Penicillin(100U/ml) + Streptomycin (100 μg/ml) • 2 mM L-glutamine • Non-essential aminoacids 1 % • 10 mM β glicero-phosphate • 50 μg ascorbic acid • 10 nM dexamethasone	• DMEM glucose :F12 HAM(1:1) • 10 % fetal calf serum • Penicillin(100U/ml) + Streptomycin (100 μg/ml) • 2 mM L-glutamine • Non-essential aminoacids 1 % • 10 mM β glicero-phosphate • 50 μg ascorbic acid • 10 nM dexamethasone • 3 ng/ml BMP2 • 2 ng/ml TGF β1