Fig. 2. Optimization of the reaction temperature and MgSO4 concentration for the blaVIM-2, blaIMP-1, and blaOXA-23 loop-mediated isothermal amplification (LAMP) assays. (A-C) Ten ng of DNA was used as a template, and the reaction was performed for 1 hr at the temperatures indicated at the top. The 'DW' reaction was performed at 62℃. (D-F) Ten ng DNA was used as a template, and the reaction was performed for 1 hr with MgSO4 concentrations indicated at the top. LAMP reaction was performed at 62℃. 'DW' means distilled water used in replace of the template DNA as a negative control. The reaction products were loaded onto 1.5% agarose gel for analysis.
