Table 1. Definition of the cutoff values* for basophil activation markers according to four basophil preparation methods and three basophil gating strategies.
| DNAUP basophils | DNAP basophils | DAUP basophils | DAP basophils | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD203c | DP | CD63 | CD203c | DP | CD63 | CD203c | DP | CD63 | CD203c | DP | CD63 | |
| Gate 1 | 1.8 | 0.6 | 5.6 | 76.4 | 3.8 | 8.8 | 5.1 | 0.3 | 4.5 | 70.0 | 6.2 | 10.9 |
| Gate 2 | 5.2 | 3.5 | 10.4 | 70.8 | 4.9 | 13.9 | 3.7 | 0.8 | 10.0 | 56.6 | 6.1 | 14.8 |
| Gate 3 | 2.0 | 0.6 | 2.9 | 110.5 | 4.6 | 8.4 | 1.7 | 0.3 | 4.3 | 91.3 | 7.8 | 10.9 |
Data are presented as percentages.
*The cutoff value for an activation marker was defined as the average of its expression percentages+(3×SD) in negative control sera (n=20), which were measured on the CD63/CD203c plot of gated basophils (Fig. 1, right plot).
Abbreviations: DNAUP, non-atopic donor unprimed; DNAP, non-atopic donor primed; DAUP, atopic donor unprimed; DAP, atopic donor primed basophils; DP, double positive (CD63+CD203c+).