Table 2. Comparison of assay sensitivity (%) among basophil activation markers according to four basophil preparation methods and three basophil gating strategies in positive control sera (n=21-24)^*.

	DNAUP basophils			DNAP basophils			DAUP basophils			DAP basophils
	CD203c	DP	CD63	CD203c	DP	CD63	CD203c	DP	CD63	CD203c	DP	CD63
Gate 1	95.2	9.5	0.0	8.3	79.2	79.2	95.5	45.5	13.6	57.1	100	100
Gate 2	76.2	9.5	4.8	0.0	75.0	66.7	95.5	27.3	13.6	52.4	100	100
Gate 3	95.2	4.8	4.8	0.0	75.0	75.0	100	50.0	13.6	9.5	100	100

Data are presented as percentages.

^*See Table 1 for the cutoff values of each marker, which were used to discriminate the expression percentages as positive or negative. The assay specificity using such a cutoff value can be regarded as 99%.

Abbreviations: D_NA^UP, non-atopic donor unprimed; D_NA^P, non-atopic donor primed; D_A^UP, atopic donor unprimed; D_A^P, atopic donor primed; DP, double positive (CD63+CD203c+).