Table 1.

Conserved expression features between sponge and mammalian RBPs

				Ephydatia
Mammals					Expression value
Gene symbol	Positive/negative regulator of ESC renewal	mESC-specific in ref. 32	OrthoMCL group	Transcript(s) in the same OrthoMCL group	Archeo	Other	Choano	Differential expression cluster (Fig. 2)
MBNL1	Negative*	No	OG5_132352	m.6274	29.835	164.2	139.68	5
Pum1	Negative†	Yes	OG5_126869	m.145795	2.27	0.575	0.275	3
ESRP1	Negative‡	Yes	OG5_130871	m.33711	54	9.835	4.305	3
Nat1	Negative	Yes	OG5_132868	m.25377	66.6	34.685	12.84	3
RBFOX2	Positive§	Yes	OG5_135623	m.28075	178.615	60.585	55.48	3
ADAR	Positive¶	No	OG5_138421	m.34972	3.36	2.2	2.17	No diff. exp.
SON	Positive#	No	OG5_133903	m.27671	19.735	5.665	4.065	3
Zcchc11	Positive	No	OG5_128508	m.33121	17.135	4.05	2.49	3
Zcchc6	Positive	No	OG5_128508	m.33713	14.455	1.58	0.555	3
Mettl3	Positive	No	OG5_129618	m.23877	26.545	12.54	13.2	3
Mettl4	Positive	No	OG5_132677	m.28272	8.62	3.65	2.945	3
Thoc2	Positive||	No	OG5_129528	m.14726	116.27	13.425	7.13	3
Thoc5	Positive**	No	OG5_130349	m.16805	97.675	17.915	11.99	3
Ptbp1	Positive	Yes	OG5_127719	m.11907	132.735	54.79	16.635	3

No diff. exp., not differentially expressed.

^*Lower in mESC than in several differentiated tissues (61).

^†Increases during early differentiation of mESC and then comes back to the initial level (62).

^‡Highly enriched in mESC (63).

^§Expressed in Oct4+, Nanog+, and Sox2+ mESCs, not in Oct4-negative fibroblasts (64).

^¶No significant changes during hESC differentiation (65).

^#Increases during hESC reprogramming and decreases during ESC differentiation (66).

^||Decreases during mESC differentiation (67).

^**Decreases during mESC differentiation (67).