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. 2015 Dec 7;112(51):15642–15647. doi: 10.1073/pnas.1519644112

Fig. 3.

Fig. 3.

The CREB/CRTC pathway promotes M2 function via induction of KLF4. (A) Transient assay of HEK293T cells showing effects of FSK on KLF4 reporter activity. Cotransfection with ACREB or CRTC2 indicated. (B) ChIP (26) assay of pCREB and CRTC2 recruitment to the KLF4 promoter in BMMs exposed to PGE2. (C) Effect of PGE2 exposure for various times on KLF4 mRNA amounts in BMMs from wild-type, CREB MKO, and CRTC2/3 DKO mice. (D and E) Effect of lentiviral KLF4 expression on M2 macrophage marker mRNA amounts (D) and Arg1 protein level (E) in wild-type and CREB MKO BMMs exposed to IL-4 and PGE2. *P < 0.05 and **P < 0.01.