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. 2015 Dec 7;112(51):15654–15659. doi: 10.1073/pnas.1511285112

Fig. 5.

Fig. 5.

Tmem178 deletion worsens inflammatory bone loss. (A) Quantification of TRAP+ OCs in WT and Tmem178−/− cells following the addition of 100 ng/mL LPS or 10 ng/mL TNF-α on day 2 of OC culture. ***P < 0.001, **P < 0.01. (B) Representative microCT 3D reconstructions of calvaria from WT and Tmem178−/− mice receiving 100 µg of supracalvarial LPS. n = 10 per genotype. (C) Quantification of the percent area resorbed on the calvaria from B. **P < 0.01. (D) Quantification of OC surface per bone surface (Oc.S./B.S.) in B. **P < 0.01. (E) Representative microCT 3D reconstructions of knees from WT and Tmem178−/− mice after K/BxN serum transfer arthritis. n = 10 per genotype. (F) Quantification of bone volume remaining at the knee in mice in E. **P < 0.01, n = 10 per genotype. (G) Quantification of Oc.S/B.S. from E. **P < 0.01, n = 10 per genotype. (H) Q-RT PCR analysis of Tmem178 mRNA expression in human CD14+ cells cultured in osteoclastogenic medium for 2 d before addition of healthy control (HC) or sJIA plasma. **P < 0.01, *P < 0.05. (I) Quantification of area covered by TRAP + OCs cultured with HC plasma or sJIA plasma. *P < 0.05. Triplicate wells were counted. Representative of two independent experiments. (J) Representative images of TRAP+ OCs in I. (K) Quantification of OC area from murine BMMs expressing pMX or Tmem178 and treated with HC or sJIA plasma. **P < 0.01.