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. 2015 Dec 7;112(51):15654–15659. doi: 10.1073/pnas.1511285112

Fig. S5.

Fig. S5.

(A) Representative immunofluorescence images of NFATc1 (red) in WT and Tmem178−/− BMMs cultured with 10 ng/mL M-CSF and 50 ng/mL RANKL for the indicated days. DAPI (blue) was used as nuclear stain. (B) Western blot analysis of NFATc1 nuclear translocation in response to 50 ng/mL RANKL in WT and Tmem178−/− preOCs for the indicated time points. Lamin B is shown as loading control, representative of three experiments. (C) Western blot analysis of NFATc1 nuclear translocation in response to 50 ng/mL RANKL in WT cells expressing empty vector pMX or Tmem178-HA for the indicated time points. Histone 3 is shown as loading control, representative of three experiments.