Fig. 1.

Genetic mapping and rescue confirming that the prd-1 gene is NCU07839. (A) The genetic map location of prd-1 on linkage group III near to the centromere (31). (B) Genetic outcome of three point mapping cross between parental strains with chromosomes marked blue and black. Numbers of progeny and rates of recombination are noted; no double cross-over events were achieved. (C) Cassette that was transformed into prd-1; ras-1[bd]; mus-51::bar⁺ background to replace prd-1 with NCU07839, and race tube confirmation of WT period length and phenotype in the transformant compared with prd-1; ras-1[bd] and ras-1[bd] controls. Three independent progeny are shown.