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. 2015 Dec 8;112(51):15749–15754. doi: 10.1073/pnas.1512296112

Fig. 1.

Fig. 1.

Synaptic ZnT3-dependent zinc inhibits AMPAR EPSCs in DCN parallel fiber synapses via a postsynaptic mechanism. (A) Schematic of the experimental setup for electrophysiological experiments in cartwheel cells. In this figure, AMPAR EPSCs were recorded from cartwheel cells and evoked by parallel fiber stimulation (PF EPSCs). (B) Representative PF EPSCs before and after ZX1 application. (C) Time course of PF EPSC amplitude before and after ZX1 application (PF EPSC amplitude, 15–20 min after ZX1 application: 140.22 ± 7.66% of baseline, n = 7, P < 0.01). (D) Representative PF EPSCs in response to two stimuli 20 ms apart: before and after ZX1 application. (E) Summary graph of paired-pulse ratio (n = 7, P = 0.36 for control vs. ZX1). (F) Summary graph of normalized 1/CV2 (n = 7, P = 0.01 for second pulse vs. first pulse; n = 7, P = 0.95 for control first pulse vs. ZX1 first pulse). (G) Representative PF EPSCs in control, after tricine, and after tricine and ZX1 application. (H) Time course of PF AMPAR EPSC amplitude before, after tricine, and after tricine and ZX1 application (PF EPSC amplitude: 15–20 min after tricine application: 100.07 ± 3.35% of baseline, n = 5, P = 0.97; 15–20 min after tricine and ZX1 application: 146.51 ± 7.20% of baseline, n = 5, P < 0.01). (I) Representative PF EPSCs from ZnT3WT and ZnT3KO mice before and after ZX1 application. (J) Time course of PF EPSC amplitude from ZnT3WT and ZnT3KO mice before and after ZX1 application (PF EPSC amplitude, 15–20 min after ZX1 application: ZnT3WT: 147.02 ± 8.82% of baseline, n = 5, P < 0.01; ZnT3KO: 94.69 ± 6.85% of baseline, n = 5, P = 0.16; ZnT3WT vs. ZnT3KO: P < 0.01). Values represent mean ± SEM. For details of statistical tests and detailed values shown in main figures, see SI Materials and Methods.