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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 31;1007:121–126. doi: 10.1016/j.jchromb.2015.10.012

Figure 2.

Figure 2

LC-ESI+-MS/MS analysis of NNN in a smokers’ urine. The upper trace represents NNN in urine (shaded peak). The lower trace is the internal standard [13C6]NNN. The middle trace shows no significant formation of [pyridine-D4]NNN in the artifact test using added [pyridine-D4]nornicotine as the monitor amine. B. Analysis of NNAL in the same urine sample. The upper trace is NNAL, the E- and Z-rotamers eluting at 8.65 and 9.06 min, respectively, while the lower trace is the internal standard [13C6]NNAL. The column effluent was diverted to waste for the first 6 min, then to the mass spectrometer.