Figure 2.

Phenformin treatment inhibits growth in H1792 and H358 xenograft models. A. Tumor growth curve of H1792 and H358 xenografts. H1792 and H358 xenografts were treated with phenformin 50 mg/kg or vehicle for 25 days after tumor volume reached an average of 200 to 300 mm³. Data are presented as means ± SD, n = 6, **P < 0.01 versus vehicle group. B. Body weight changes in phenformin and vehicle treated mice. Data are presented as means ± SD, n = 6. C. Representative images of Ki-67 staining in H1792 and H358 xenografts. Scale bar represents 50 μm. Quantification of proliferating cells by Ki-67 staining in H1792 and H358 xenografts. Data are presented as means ± SD, n = 6, **P < 0.01 versus vehicle group. D. Biochemical analysis of ERK, AKT and Caspase 3 in protein extract of H1792 and H358 xenografts. Protein loading was normalized by GAPDH. Three independent samples were evaluated.